UJI AKTIVITAS EKSTRAK Chondrus sp SEBAGAI ANTIBAKTERI Escherichia coli.
Kode Repository :SKB06/YUN/23
NPM :061118030
Nama :Yunus
Pembimbing 1 :-Dra. Tri Saptari Haryani. M.Si.
Pembimbing 2 :-Dra. Triastinurmiatiningsih, M.Si
Abstrak :-Yunus. NPM: 061118030. Title: Chondrus sp. Activity Test as Antibacterial
Escherichia coli. Guided By: Dra. Tri Saptari Haryani, M.Si. and Dra.
Triastinurmiatiningsih, M.Si.
Escherichia coli is a bacterium that causes diarrheal diseases or
gastrointestinal infections in humans that often occur in various geographies of
the world. One of the treatments of bacterial infections can be treated with the use
of antibiotics. Antibiotics can stop the growth or kill the bacteria that cause the
infection. Bacterial resistance to antibiotics is a problem that can occur due to the
unwise and rational use of antibiotics, where bacteria are no longer sensitive to
some groups of antibiotics to treat infectivesi.
The purpose of this study was to analyze the antibacterial activity of 96%
ethanol extract Chondrus sp. against the growth of Escherichia coli as one of the
causes of diarrhea. Antibacterial testing of Chondrus sp. ethanol extract begins
with the MIC (Minimum Inhibitory Concentration) Test through the agar dilution
method and antibacterial test using the Disc Diffusion method by measuring the
Width of Inhibition. MIC test of ethanol extract Chondrus sp. with concentrations
of 10%, 20%, 30%, 40% and 50%. Meanwhile, the Inhibitory Power Width Test
was carried out on concentrations of 50%, 60%, and 70%. Control negatif using
sterile aqueous and kpositive ontrol using Amoxicillin 10 mg/L.
The results showed that Chondrus sp. extract has secondary metabolites
that can inhibit the growth of Escherichia coli bacteria. MIC test of 96% ethanol
extract Chondrus sp. against the growth of Escherichia coli is at a concentration
of 50%. Meanwhile, the results of antibacterial tests at a concentration of 70%
showed the greatest activity in inhibiting the growth of Escherichia coli compared
to other concentrations.