• Kode Repository : SKF95/SIT/22
• NPM : 066117223
• Nama : Siti Undari Astriyani
• Pembimbing 1 : -Apt.Dra.Ike Yulia W.,M.Farm
• Pembimbing 2 : Rima Dewi Sulistiyono, S.Si.,M.Si
• Abstrak : -UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN SAGE (Salvia officinalis L.) TERHADAP BAKTERI Staphylococcus aureus Siti Undari Astriyani, Ike Yulia W, dan Fitria Dewi Sulistiyono Program Studi Farmasi, Fakultas MIPA – Universitas Pakuan Email: sitiundariastr@gmail.com ABSTRAK Tanaman sage adalah tanaman obat yang bernilai tinggi, yang banyak digunakan dalam pengobatan tradisional karena mempunyai aktivitas antioksidan, antibakteri, antijamur, antiinflamasi, antiviral, fungisida, virustatik, astringen dan antialergi. Tujuan penelitian ini adalah menentukan aktivitas antibakteri ekstrak daun sage terhadap bakteri Staphylococcus aureus dengan menggunakan metode Microwave Assisted Extraction. Ekstraksi dilakukan menggunakan microwave oven dengan daya 800watt selama 8 menit. Pengujian Konsentrasi Hambat Minimum (KHM) pada ekstrak yaitu pada konsentrasi 1.25; 2.5; 5.0; dan 10.0%. Sedangkan Konsentrasi untuk uji Lebar Daya Hambat (LDH) yaitu 10, 20, dan 30%. Hasil penelitian menunjukkan bahwa ekstrak daun sage memiliki aktivitas antibakteri pada bakteri Staphylococcus aureus. Aktivitas antibakteri daun sage yaitu pada konsentrasi ekstrak 10% berdasarkan uji KHM. Konsentrasi yang paling efektif sebagai antibakteri yaitu ekstrak daun sage konsentrasi 30% berdasarkan uji LDH dengan diameter hambat 13,46 mm yang termasuk kategori sangat kuat. Kata Kunci: Antibakteri, Daun Sage, MAE, Staphylococcus aureus ABSTRACT Sage is a medicinal plant with high value, which is widely used in traditional medicine because it has antioxidant, antibacterial, antifungal, anti-inflammatory, antiviral, fungicidal, viral static, astringent and anti-allergic activities. The purpose of this study was to determine the antibacterial activity of sage leaf extract against Staphylococcus aureus bacteria using the Microwave Assisted Extraction method. Extraction was carried out using a microwave oven with a power of 800 watts for 8 minutes. Testing the Minimum Inhibitory Concentration (MIC) on the extract, namely at a concentration of 1.25; 2.5; 5.0; and 10.0%. While the concentrations for the Inhibitory Width test are 10, 20, and 30%. The results showed that sage leaf extract had antibacterial activity against Staphylococcus aureus bacteria. The antibacterial activity of sage leaves is at a concentration of 10% extract based on the MIC test. The most effective concentration as an antibacterial was sage leaf extract with a concentration of 30% based on the LDH test with an inhibitory diameter of 13.46 mm which was categorized as very strong. Keywords: Antibacterial, Sage Leaf, MAE, Staphylococcus aureus
• Program Studi : Farmasi