Uji Aktivitas Antibakteri Ekstrak Etil Asetat Daun Salam (Syzygium polyanthum) Terhadap Bakteri Staphylococcus aureus Dan Escherichia coli
Kode Repository :SKF95/NUR/22
NPM :066117200
Nama :Siti Nurlaela
Pembimbing 1 :-Dr. Oom Komala, MS
Pembimbing 2 :-apt. Drs. Almasyhuri, M.Si
Abstrak :-UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT DAUN SALAM (Syzygium polyanthum) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli
Siti Nurlaela1*, Oom Komala2, Almasyhuri3
1,2.3 Program Studi Farmasi, FMIPA Universitas Pakuan,Jalan Pakuan PO BOX 452, Bogor 16143
*Email korespondensi: mellasiti12@gmail.com
Diterima: Direvisi: Disetujui:
ABSTRAK
Infeksi merupakan salah satu penyakit dan masalah kesehatan dalam masyarakat. Salah satu penyebabnya adalah bakteri. Bakteri Staphylococcus aureus dan Escherichia coli merupakan bakteri patogen yang paling banyak menyerang manusia. Daun salam (Syzygium polyanthum) mengandung senyawa kimia lain yang memberikan aktivitas antibakteri seperti alkaloid, tannin, flavonoid, dan triterpenoid. Penelitian ini betujuan untuk mengetahui konsentrasi yang paling aktif terhadap Escherichia coli dan Staphylococcus aureus. Simplisia daun salam diekstraksi menggunakan metode Microwave Assisted Extraction (MAE) pelarut etil asetat. Metode ekstrak diuji konsentrasi hambatan minimum pada konsentrasi 22,5%, 25% dan 30% dengan menggunakan metode dilusi agar. Uji aktivitas antibakteri menggunakan metode difusi cakram pada konsentrasi 30%, 35%, dan 40%. Kontrol positif yang digunakan ciprofloxacin 100 ppm dan kontrol negatif tween 80 10%. Hasil penelitian menunjukkan bahwa ekstrak daun salam mempunyai daya hambat terhadap bakteri Escherichia coli dan Staphylococcus aureus. aktivitas antibakteri dengan rata-rata lebar daya hambat terhadap Escherichia coli dengan konsentrasi 30% sebesar 2,95 mm, 35% sebesar 3,99 mm dan 40% sebesar 6,02 mm dan kontrol positif sebesar 9,47 mm sedangkan terhadap Staphylococcus aureus dengan konsentrasi 30% sebesar 3,19 mm, 35% sebesar 4,60 mm, dan 40% sebesar 6,12 mm dan kontrol positif sebesar 9,35 mm kontrol negatif tidak memiliki zona hambat. Kesimpulan konsentrasi ekstrak daun salam 40% lebih menghambat bakteri Escherichia coli dan Staphylococcus aureus maka semakin tinggi kosentrasi semakin besar daya hambat bakteri.
Kata kunci: Antibakteri; Bakteri Staphylococcus aureus dan Escherichia coli; Daun salam.
ANTIBACTERIAL ACTIVITY TESTING OF (Syzygium polyanthum) ETHLACETATE EXTRACT AGAINST Staphylococcus aureus and Escherichia coli
ABSTRACT
Infection is one of the diseases and health problems in society. One of the causes is bacteria. Staphylococcus aureus and Escherichia coli are the most common pathogenic bacteria that attack humans. Bay leaves (Syzygium polyanthum) contain other chemical compounds that provide antibacterial activity such as alkaloids, tannins, flavonoids, and triterpenoids. This study aims to determine the most active concentrations against Escherichia coli and Staphylococcus aureus. Bay leaf simplicia was extracted using the Microwave Assisted Extraction (MAE) method with ethyl acetate solvent. The extract method was tested for minimum inhibitory concentrations at concentrations of 22.5%, 25% and 30% using the agar dilution method. Antibacterial activity test using disc diffusion method at concentrations of 30%, 35%, and 40%. Positive control used ciprofloxacin 100 ppm and negative control tween
80 10%. The results showed that bay leaf extract had an inhibitory effect on Escherichia coli and Staphylococcus aureus bacteria. antibacterial activity with an average width of inhibition against Escherichia coli with a concentration of 30% of 2.95 mm, 35% of 3.99 mm and 40% of 6.02 mm and positive control of 9.47 mm while against Staphylococcus aureus with 30% concentration was 3.19 mm, 35% was 4.60 mm, and 40% was 6.12 mm and the positive control was 9.35 mm. The negative control had no inhibition zone. The conclusion is that the 40% concentration of bay leaf extract inhibits Escherichia coli and Staphylococcus aureus bacteria, the higher the concentration, the greater the inhibitory power of the bacteria.
Keywords: Antibacterial; Bacteria Staphylococcus aureus and Escherichia coli; Syzygium polyanthum leaf.